Source-linked AI summary
OpenSPIM - an open access platform for light sheet microscopy
Peter Pitrone, Johannes Schindelin, Luke Stuyvenberg, Stephan Preibisch, Michael Weber, Kevin W. Eliceiri, Jan Huisken, Pavel Tomancak
TL;DR
SPIM requires higher acquisition throughput for proper interpretation, while sample drift remains a recurring time-lapse problem. The paper presents an OpenSPIM platform combining imaging hardware, reconstruction, fusion, and analysis, with developmental imaging results and planned design extensions.
Problem
Higher SPIM acquisition throughput is necessary for proper interpretation, and sample drift is a notorious problem in time-lapse acquisitions.
Method
The paper builds an OpenSPIM platform that combines a fast, sensitive camera with three-dimensional imaging, reconstruction, fusion, and further analysis, emphasizing open development.
Results
The system captures developmental dynamics from germband extension through late embryogenesis, including nervous-system morphogenesis and Csp expression.
Takeaways & Limitations
OpenSPIM is intended as an accessible, openly developed instrument for life scientists studying dynamic phenomena.
Takeaways & Limitations
The current OpenSPIM design is limited to one-sided illumination and detection, although extensions toward more advanced SPIM designs are intended.
Abstract
from arXiv · showhide
Light sheet microscopy promises to revolutionize developmental biology by enabling live in toto imaging of entire embryos with minimal phototoxicity. We present detailed instructions for building a compact and customizable Selective Plane Illumination Microscopy (SPIM) system. The integrated OpenSPIM hardware and software platform is shared with the scientific community through a public website, thereby making light sheet microscopy accessible for widespread use and optimization to various applications.
Authors contributions
The authors divided responsibilities across microscope design, software implementation, image processing, data collection, supervision, and manuscript preparation.
- P.P. designed and built the microscope, collected data, analyzed images, and wrote the paper.
- J.S. and L.S. designed and implemented the software, while S.P. assisted with image-data processing.
- M.W. collected data and designed the SolidWorks concepts.
- K.E. and J.S. co-supervised the software component, while J.H. designed the setup.
- P.T. conceived and supervised the project, and all authors contributed to the work.